Publication:

ACID-INDUCED VISCOSITY COMPLEX對耐力運動之脂肪與醣類代謝的影響

Acid-induced viscosity (acid I-V) complex可能可以減緩健康受試者在攝取高醣類後血糖上升反應。本研究目的為探討acid I-V對耐力運動期間醣類與脂肪代謝的影響。共9位健康志願者參與本次研究，每位受試者各進行4次測試，每次測試於運動前3小時分別攝取葡萄糖飲料(GLU組)，acid I-V+葡萄糖飲料（包含alginate, citrate,與 insoluble calcium，Acid I-V組），nutrious (不含alginate之膳食纖維)+葡萄糖飲料(Nutrious 組），與空腹(FAST組)，測試用葡萄糖飲料均含75克葡萄糖，隨後以固定式腳踏車運動(50％VO2max) 60分鐘。以滯留針於空腹，餐後階段（15分鐘，30分鐘，60分鐘，90分鐘，120分鐘，150分鐘，180分鐘）與運動期間（15分鐘，30分鐘，45分鐘，60分鐘）自肘靜脈各採集10 ml血液樣本，並於同時間點以氣體分析儀測量VO2與VCO2，計算醣類與脂肪氧化率，分析血漿葡萄糖、insulin、C-Peptide、triglyceride、non-esterified fatty acid、glycerol、3-hydroxybutyrate、uric acid、NH3、lactate濃度。GLU，Acid I-V，與Nutrious組餐後血糖與胰島素反應顯著高於FAST組，但各組間血糖與胰島素曲線下面積無顯著差異（GLU: 626± 611.4, Acid I-V: 478±486.9, Nutrious: 470 ± 684.2, FAST: 725±689 arbitrary unit）。FAST組non-esterified fatty acid濃度於餐後30、60、90分鐘顯著高於Acid I-V組；餐後60、90、120分鐘顯著高於GLU組；餐後120、180分鐘顯著高於Nutrious組。FAST組 glycerol濃度於餐後90、120、150分鐘顯著高於其他三組，但各處置間non-esterified fatty acid及glycerol濃度曲線下面積於餐後與運動階段均無顯著差異，各組碳水化合物與脂肪氧化率亦未無顯著差異。本研究顯示，雖然acid I-V可減緩餐後升糖與胰島素反應，但對於隨後耐力運動期間之醣類與脂肪代謝並無顯著影響。

The acid-induced-viscosity complex (acid I-V） may attenuate the postprandial glycemic response to a glucose challenge in healthy subjects. The purpose of the present study was to examine the effect of acid I-V mixed on carbohydrate and fat metabolism during endurance exercise. Nine healthy volunteers participated in this study. Every subject performed 4 trials. The subjects ingested glucose drink (GLU), acid-induced viscosity complex (containing alginate, citrate, and insoluble calcium) with glucose drink (Acid I-V), nutrious with glucose drink（Nutrious）, or fasting (FAST) 3 hours before exercising on a cycle ergometer at 50%VO2max for 60 min. The test drinks contained 75 g glucose. An aliquot of 10 ml blood samples were obtained at fasting, during postprandial period (15, 30, 60, 90, 120, 150, 180 min) and during exercise (15, 30, 45, 60 min） via a cannula. Gas analysis was performed at the same time points to calculate carbohydrate and fat oxidation rates. Plasma concentrations of glucose, insulin, C-peptide, triglyceride, non-esterified fatty acid, glycerol, 3-hydroxybutyrate, uric acid, NH3, and lactate were measured. The glycemic and insulinemic responses in the postprandial periods were higher in GLU, Acid I-V, and Nutrious trials than FAST. However, the incremental areas under the curve of glucose and insulin were not significantly different among the 4 trials (GLU: 626± 611.4, Acid I-V: 478±486.9, Nutrious: 470 ± 684.2, FAST: 725±689). FAST trial had significantly higher plasma non-esterified fatty acid concentration at 30, 60, 90 min postprandial than Acid I-V; at 60, 90, 120 min postprandial than GLU; and 120 and 180 min postprandial than Nutrious. FAST trial showed significantly higher plasma glycerol concentration than the other 3 trials at 90, 120, and 150 min postprandial. However, the incremental areas under the curve of non-esterified fatty acid and glycerol were not significantly different among the 4 trials. The oxidation rates of carbohydrate and fat were also similar among the 4 trials during the postprandial and exercise periods. The results of this study suggested that although acid I-V could reduce postprandial glycemic and insulinemic responses, it had no effect on carbohydrate and fat metabolism in endurance exercise.