呂學冠Lu, Hsuen-Kuan2017-02-222025-07-302017-02-222017-02-22https://ir.ntus.edu.tw/handle/987654321/70642學位類別：碩士校院名稱：國立臺灣體育學院系所名稱：體育研究所畢業學年度：93年論文頁數：125頁　　本研究旨在探討運動員吸煙對過氧化傷害的影響。實驗以參加2004年全國大專盃跆拳道比賽之跆拳道選手為受試對象，受試者分成兩組，一組為無吸煙習慣之跆拳道選手、一組為有吸煙習慣之跆拳道選手，分別採集比賽前與比賽後之血液樣本進行生化分析，分析項目包括：（1）脂質過氧化傷害指標：丙二醛（malondialdehyde, MDA）；（2）血液抗氧化酵素指標：超氧化物岐化酶（superoxide dismutase, SOD）、麩胱甘肽過氧化酶（glutathione peroxides, GPx）；（3）骨骼肌破損指標：肌酸激酶（creatine kinase, CK）、乳酸去氫酶（lactate dehydrogenase, LDH）； （4）心肌破損指標：MB型肌酸激酶（creatine kinase polypeptide subunit MB, CK-MB）； （5）肝細胞破損指標：谷草轉氨酶（glutamic oxaloacet transaminase, GOT）、谷丙轉氨酶（glutamic-pyruvic teansaminase, GPT）； （6）血球相關指標：紅血球數（red blood cell, RBC）、血紅素（hemoglobin, Hgb）及血比容（hematocrit, Hct）。研究以t-test比較比賽前、後，各生化值之變異，結果以平均值±標準誤表示。各研究數據均以 SPSS 10.0 for windows軟體進行統計分析，顯著差異機率值定為P<.05。結果顯示，比賽前所有血液生化分析數值皆已超過正常值範圍，無論組內或組間均未出現顯著之差異。對照組比賽前後組間之血球分析數值，Hct有顯著差異（p=.017）。 　　本研究獲得以下主要結論：1.比賽前之集訓期已經造成選手運動過氧化傷害；2.一次跆拳道比賽不會對選手造成顯著的過氧化傷害；3.有吸煙習慣之運動員比賽後吸煙對運動過氧化傷害沒有加成之效應；4.跆拳道比賽前之集訓期能有效的提高運動員身體抗氧化酵素酶SOD與GPx的活性，提高身體抵禦自由基的能力。Abstract 　　The purpose of this study was to investigate the effects of cigarette smoking on per-oxidation injuries on athletes of the Tae Kwon Do. Volunteers Tae Kwon Do players from 2004 National College Games were assigned to No-smoke group and Smoking group. Blood samples were taken before and after competition. We used malondialdehyde (MDA) as an indicator of lipid per-oxidation, the superoxide dismutase (SOD) and glutathione peroxides (GPx) as indicators of the activity of anti-oxidation enzymes, the creatine kinase (CK) and the lactate dehydrogenase (LDH) appearing in the serum as the indicators of muscular damage, the creatine kinase polypeptide subunit MB (CK-MB) appearing in the serum as an indicator of myocardium damage, the glutamic oxaloacet transaminase (GOT) and glutamic-pyruvic teansaminase (GPT) appearing in the serum as indicators of liver cell damage, and the blood red blood cell (RBC), hemoglobin (Hgb) and hematocrit (Hct) count as the indicators of hematocyte. Statistical analyses were performed on SPSS (v.10.0). Results are expressed as mean ± standard error (SE). The statistical significance of data was assessed by t-test. Only a probability level (p) of significance of p < .05 was considered significant. The results showed that all the mean variables of each blood biochemistry analysis were out of the normal range before competition and there was no significant difference between trails and within groups. Hct variables of No-smoke group showed significant difference within groups (p=.017). 　　The conclusions of this study are as followings：1) training period has caused the per-oxidation injuries on athletes of the Tae Kwon Do; 2) there was no significant per-oxidation injuries after the competition of the Tae Kwon Do; 3) cigarette smoking no cause more serious damage of per-oxidation injuries after the competition of the Tae Kwon Do; and 4) training has the protective effect on per-oxidation injuries by elevating the activity of SOD and GPx.目　次 第壹章　緒論…………………………………………………………………………1 　第一節　研究動機…………………………………………………………………1 　第二節　研究目的…………………………………………………………………4 　第三節　研究假設…………………………………………………………………4 　第四節　研究範圍…………………………………………………………………5 　第五節　名詞操作性定義…………………………………………………………5 第貳章　文獻探討……………………………………………………………………8 　第一節　香煙………………………………………………………………………8 　第二節　自由基……………………………………………………………………13 　第三節　技擊運動：跆拳道………………………………………………………19 　第四節　吸煙與自由基的關係……………………………………………………25 　第五節　吸煙對運動表現的影響…………………………………………………26 　第六節　運動與過氧化傷害………………………………………………………28 　第七節　運動吸煙與過氧化傷害…………………………………………………35 第叁章　研究方法與步驟……………………………………………………………36 　第一節　研究對象…………………………………………………………………36 　第二節　實驗設計與分析…………………………………………………………36 　第三節　實驗步驟…………………………………………………………………42 　第四節　資料處理…………………………………………………………………43 第肆章　結果…………………………………………………………………………46 　第一節　有吸煙習慣者運動後一次吸煙處理對脂質過氧化傷害之影響………47 　第二節　有吸煙習慣者運動後一次吸煙處理對抗氧化酵素產生之影響………49 　第三節　有吸煙習慣者運動後一次吸煙處理對骨骼肌肉細胞破損之影響……53 　第四節　有吸煙習慣者運動後一次吸煙處理對心肌細胞破損之影響…………57 　第五節　有吸煙習慣者運動後一次吸煙處理對肝細胞破損之影響……………59 　第六節　有吸煙習慣者運動後一次吸煙處理對血球之影響……………………63 第伍章　討論…………………………………………………………………………69 　第一節　有吸煙習慣者運動後一次吸煙處理對脂質過氧化傷害之影響………69 　第二節　有吸煙習慣者運動後一次吸煙處理對抗氧化酵素產生之影響………71 　第三節　有吸煙習慣者運動後一次吸煙處理對骨骼肌肉細胞破損之影響……73 　第四節　有吸煙習慣者運動後一次吸煙處理對心肌細胞破損之影響…………74 　第五節　有吸煙習慣者運動後一次吸煙處理對肝細胞破損之影響……………75 　第六節　有吸煙習慣者運動後一次吸煙處理對血球之影響……………………76 第陸章　結論與建議…………………………………………………………………77 　第一節　結論………………………………………………………………………77 　第二節　建議………………………………………………………………………78 參考文獻………………………………………………………………………………79515325 bytesapplication/pdf跆拳道;香煙;自由基;過氧化傷害Tae Kwon Do;free radical;Smoke;per-oxidationthesis