張振崗Chang, Chen-Kang2017-02-272025-07-302017-02-272017-02-27https://ir.ntus.edu.tw/handle/987654321/70986學位類別：碩士校院名稱：國立台灣體育大學系所名稱：競技運動學系碩士班學號：19604020畢業學年度：98年論文頁數：66頁　　支鏈胺基酸和精胺酸對於人體有許多生理功能，補充支鏈胺基酸可能可以增加胰島素反應，進而促進肌肉肝醣回補，可能可以降低骨骼肌中的蛋白質分解；補充精胺酸可能可以促進血管擴張降低運動時血液中所堆積的氨和乳酸，進而延緩肌肉疲勞，增進運動表現。本研究目的為探討補充支鏈胺基酸與精胺酸，對高強度間歇運動後之恢復期與及後續運動表現的影響，並探討其可能的生化機轉。本研究以9名男性大學角力選手為受試者，每名受試者皆以隨機順序進行3個trial，每個trial包含3次運動，每次運動包含3階段，受試者每階段於腳踏車測功計重覆10秒全力衝刺及20秒休息，在全力衝刺期間阻力設為0.1 kp/kg，每階段包含4次的間歇性運動型式，2分鐘階段間休息1分鐘。第一次運動後進行1小時的恢復期，在第二次運動後進行2小時的恢復期。而在第二次運動後立即補充1g/kg碳水化合物＋0.1 g/kg 精胺酸＋0.1 g/kg 支鏈胺基酸(GLU+AA trial)、1.2 g/kg 碳水化合物(GLU trial)、或安慰劑(PLA trial)。血液採集和氣體分析於早餐食用前、第一次運動前、第一次運動後0分鐘、30分鐘、60分鐘、第二個階段運動後0、30分、60分、90分、120分鐘、第三個運動階段後。氣體樣本分析項目為碳水化合物氧化率、脂肪氧化率；血液樣本分析項目為血漿中葡萄糖、胰島素、非酯化脂肪酸、甘油、乳酸、氨、肌酸激酶、乳酸脫氫酶。結果顯示三個trial的運動表現並沒有顯著的差異，各trial在各次運動總平均功率無顯著差異（EX1：GLU+AA trial 64.24 ±4.14 W/kg；GLU trial 63.90 ±3.82W/kg；PLA trial 61.97 ±3.33W/kg，EX2：GLU+AA trial 63.48 ±5.54 W/kg；GLU trial 61.05 ±4.59 W/kg；PLA trial 61.41 ±4.84 W/kg；EX3：GLU+AA trial63.85 ±7.09 W/kg；GLU trial 60.89 ±4.42 W/kg；PLA trial 59.27 ±4.15 W/kg），三階段總最大功率亦無顯著差異。補充飲料後第60、90分鐘GLU+AA trail碳水化合物氧化率顯著高於PLA trial，GLU trial第60分鐘顯著高於PLA組。各運動階段後碳水化合物曲線下面積，3個trial間無顯著差異。補充飲料後第60、90分鐘GLU+AA trial脂肪氧化率顯著低於PLA trial，而GLU trial第90、120分鐘顯著低於PLA trial，各運動階段後脂肪氧化率曲線下面積，GLU+AA trial與GLU trial顯著低於PLA組。補充後第30分鐘GLU+AA trail與GLU trial血漿中葡萄糖濃度顯著大於PLA trial，第二運動階段後恢復期血漿中葡萄糖曲線下面積，GLU+AA trial與GLU trial顯著高於PLA組。補充飲料後第30、60、90分鐘GLU+AA trial 胰島素濃度顯著高於PLA trial，GLU trial第30分鐘顯著高於PLA trial。第二運動階段後恢復期血漿中胰島素曲線下面積，GLU+AA trial與GLU trial顯著高於PLA組。非酯化脂肪酸與甘油濃度為補充飲料後第90、120分鐘及第三階段運動後GLU+AA trial顯著低於PLA trial，而GLU trial亦顯著低於PLA trial。乳酸、氨、肌酸激酶與乳酸脫氫酶濃度在各trial之間則無顯著差異。研究結果顯示高強度間歇運動後補充碳水化合物可以提高血糖與胰島素濃度、碳水化合物氧化率，並降低血漿中NEFA、Glycerol濃度、脂肪氧化率，但補充支鏈胺基酸與精胺酸無加成效果，且對於後續運動表現並無顯著的影響。　　Branched-chain amino acids (BCAA) and arginine (ARG) have a wide range of physiological functions that may improve exercise performance. BCAA may stimulate the insulin response to increase muscle glycogen recovery and reduce skeletal muscle protein proteolysis. Arginine may stimulate endothelium-dependent vasodilation and reduce exercise-induced blood lactate and ammonia accumulation. The purpose of this study was to investigate the effect of BCAA and ARG supplementation on recovery after intermittent high-intensity exercise and performance in the subsequent exercise. The potential biochemical mechanisms were also explored. Nine male college wrestlers were recruited. All subjects completed 3 experimental trials in a random order. The intermittent anaerobic test was consisted of 3 rounds with 4 sets in each round. The subjects alternated 10-sec all-out exercise and 20-sec periods on a cycle ergometer. There was 1 min rest between each round. The load in the exercise period was set 0.1 kp/kg. There was a 1-hr recovery period after the first exercise test, and 2-hr recovery period after the second exercise test. After the second exercise test, the subjects consumed 1 g/kg glucose plus 0.1 g/kg arginine and 0.1 g/kg BCAA (leucine：isoleucine：valine=2：1：1)(GLU+AA trial), 1.2 g/kg glucose (GLU trial), or Placebo (PLA trial). The blood and expired gas samples were analyzed before breakfast, immediately before and after the first exercise, 30 and 60 min after the first exercise, 0, 30, 60, 90 and 120 min after the second exercise, and immediately after the third exercise. Carbohydrate and fat oxidation rates were calculated from the results of gas analysis. The plasma sample were used to measure glucose, insulin, nonesterified fatty acids (NEFA), glycerol, lactate, NH3, creatine kinase (CK), lactate dehydrogenase (LDH). The results showed that there was no difference in exercise performance among the 3 trials. Total average power was similar among the 3 trials (EX1: GLU + AA trial 64.24 ± 4.14 W / kg; GLU trial 63.90 ± 3.82W/kg; PLA trial 61.97 ± 3.33W/kg, EX2: GLU + AA trial 63.48 ± 5.54 W / kg; GLU trial 61.05 ± 4.59 W / kg; PLA trial 61.41 ± 4.84 W / kg; EX3: GLU + AA trial63.85 ± 7.09 W / kg; GLU trial 60.89 ± 4.42 W / kg; PLA trial 59.27 ± 4.15 W / kg). Total peak power was also similar among the 3 trials. GLU + AA trail had significantly higher carbohydrate oxidation rate at 60 and 90 min postprandial than PLA trial. GLU trial had significantly higher carbohydrate oxidation rate at 60 min postprandial than PLA trial. The area under the curve (AUC) of carbohydrate oxidation rate was similar among the 3 trials. GLU + AA trail had significantly lower fat oxidation rate at 60 and 90 min postprandial than PLA trial. GLU trial had significantly lower fat oxidation at 60 and 120 postprandial min than PLA trial. The AUC of fat oxidation rate was significantly lower in GLU + AA and GLU trial than PLA trial. GLU + AA and GLU trial had significantly higher plasma glucose concentration at 30 min postprandial than PLA trial. The plasma glucose AUC after the second exercise was significantly higher in GLU + AA and GLU trial than that in PLA trial. GLU + AA trail had significantly higher plasma insulin concentration at 30, 60, and 90 min postprandial than PLA trial. GLU trial had significantly higher plasma insulin concentration at 30 min postprandial than PLA trial. The plasma insulin AUC after the second exercise was significantly higher in GLU + AA trail and GLU trial than that in PLA trial. GLU + AA and GLU trial had significantly lower plasma NEFA and glycerol concentrations at 90 and 120 min after the second exercise and immediately after the third exercise than PLA trial. There were no differences in plasma lactate concentration, NH3 concentration, CK concentration, and LDH concentration among the 3 trials. The current results suggested that the supplementation of carbohydrate can increase plasma glucose and insulin concentration and carbohydrate oxidation rate,while reducing plasma NEFA and Glycerol concentrations and fat oxidation. However, BCAA and arginine supplementation showed no additional effect on substrate metabolism and the performance in the subsequent exercise after intermittent high-intensity exercise.目　次 第一章　緒論……………………………………………………………1 　第一節　研究背景……………………………………………………1 　第二節　研究目的……………………………………………………2 　第三節　研究假設……………………………………………………3 第二章　文獻探討………………………………………………………4 　第一節　運動對肌肉肝醣的影響……………………………………4 　第二節　支鏈胺基酸對運動表現的影響……………………………7 　第三節　精胺酸與運動表現的影響…………………………………12 第三章　研究方法與步驟………………………………………………15 　第一節　研究對象……………………………………………………15 　第二節　實驗設計……………………………………………………15 　第三節　運動測試……………………………………………………16 　第四節　實驗方法……………………………………………………17 　第五節　資料處理與統計分析………………………………………20 第四章　結果……………………………………………………………21 第五章　討論……………………………………………………………25 第六章　結論與建議……………………………………………………32 　第一節　結論…………………………………………………………32 　第二節　建議…………………………………………………………33 參考文獻…………………………………………………………………34438158 bytesapplication/pdf支鏈胺基酸;精胺酸;肝醣;高強度間歇運動;運動表現branched-chain amino acids;arginine;glycogen;high-intensity exercise;exercise performancethesis