Publication:
探討活化的吞噬細胞對C2C12肌肉細胞移動速度的影響

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dc.contributor.advisor方世華
dc.contributor.advisorFang, Shih-Hua
dc.creator黃淑宜
dc.creatorHuang, Shu-Yi
dc.date2011
dc.date.accessioned2017-02-27T06:48:25Z
dc.date.accessioned2025-07-30T15:30:14Z
dc.date.available2017-02-27T06:48:25Z
dc.date.issued2017-02-27T06:48:25Z
dc.description學位類別：碩士
dc.description校院名稱：國立台灣體育大學
dc.description系所名稱：競技運動學系碩士班
dc.description學號：19604012
dc.description畢業學年度：99年
dc.description論文頁數：65頁
dc.description.abstract　　骨骼肌具有自我再生的能力，透過位於肌纖維膜及基底層的肌肉前趨細胞進行增生及分化，最後形成具有功能性的肌纖維。當肌肉損傷時會產生一連串的免疫反應，嗜中性白血球、單核球及巨噬細胞會移至損傷部位，然後釋放相關的發炎物質，促進發炎反應進行，這些物質可以吸引肌肉前趨細胞移至損傷部位，使肌纖維得到修復。 　　研究指出，高濃度的腫瘤壞死因子-α (TNF-α)及介白素-6 (IL-6)會造成骨骼肌蛋白質流失及肌肉萎縮，因此，本研究擬觀察活化的吞噬細胞所分泌的TNF-α及IL-6對C2C12肌肉細胞移動能力的影響。結果發現，巨噬細胞釋放的TNF-α及IL-6會造成肌原母細胞及肌小管細胞的移動速度變慢。此外，單純外加細胞激素TNF-或IL-6同樣也會抑制肌原母細胞及肌小管細胞的移動速度，因此我們認為，TNF-α及IL-6可能與肌肉細胞的移動速度相關。 　　此外，運動時肌肉組織往往是處於較低氧氣濃度的狀態下，因此我們也觀察在低氧條件下活化的吞噬細胞培養液對肌肉細胞移動能力的影響。結果發現，低氧會減慢肌原母細胞及肌小管細胞的移動速度，而加入活化後的吞噬細胞培養液對肌原母細胞及肌小管細胞的移動抑制更為明顯。我們認為在低氧環境中，肌肉細胞的正常功能及代謝可能會跟著改變，進而影響其移動速度。另外，實驗結果也發現，無論在正常氧分壓或低氧環境下，肌小管細胞的移動速度都比肌原母細胞慢，其原因可能是因為在正常的生理過程中，肌纖維的再生能力主要是透過肌原母細胞的增生及分化來完成。
dc.description.abstract　　Skeletal muscle has the ability to regenerate fully functional myofibers by the proliferation and differentiation of myogenic precursor cells that are located in the sarcolemma and basal layers. Muscle injury triggers a series of immune responses. Neutrophils, monocytes and macrophages migrate to the injury site and release proinflammatory factors that promote acute inflammation and attract myegenic precursors migrate to the injury site and repair injured myofibers. 　　Studies indicate that high concentrations of TNF-α and IL-6 lead to a significant loss of skeletal muscle protein and muscular dystrophy. Therefore, the aim of this study was to investigate the effects of TNF-α and IL-6 secreted by activated macrophages on the migration of C2C12-derived myogenic cells. Results show that TNF-α and IL-6 secreted by activated macrophages suppressed the myoblasts and myotubes migration. Therefore, we regard that TNF-α and IL-6 may be related to the migration speed of myogenic cells. 　　Besides, muscle tissue is frequently exposed to hypoxia during exercise. Therefore, we also observed the effects of activated macrophage conditioned medium to the migration of myogenic cells under hypoxia. Results show that the myoblasts and myotubes migration was inhibited by hypoxia. In addition, activated macrophage conditioned medium further suppressed the myoblasts and myotubes migration. On the other hand, experimental results reveal that myotubes migrate slower than myobalsts under normoxia and hypoxia. This may be due to the fact that myofibers regeneration depends on myoblasts proliferation and differentiation in normal physiological processes.
dc.description.tableofcontents目　次 第一章　緒論……………………………………………………………1 　第一節　研究背景……………………………………………………1 　第二節　研究目的……………………………………………………2 　第三節　名詞解釋……………………………………………………3 第二章　文獻探討………………………………………………………5 　第一節　肌肉細胞的形成與分化……………………………………5 　第二節　肌肉損傷後的修復及再生…………………………………7 　第三節　巨噬細胞與細胞激素對損傷肌肉的作用…………………10 　第四節　不同的氧分壓對肌肉細胞的影響…………………………13 第三章　研究方法與步驟………………………………………………15 　第一節　實驗設計……………………………………………………15 　第二節　實驗材料與方法……………………………………………17 　　壹、細胞培養………………………………………………………17 　　（一）細胞株………………………………………………………17 　　（二）細胞繼代……………………………………………………17 　　（三）細胞保存方式………………………………………………18 　　（四）肌原母細胞分化成肌小管細胞的方法……………………18 　　貳、Eosin Y染色法…………………………………………………18 　　参、活化吞噬細胞RAW264.7培養液與C2C12細胞共同培養的方式……19 　　肆、C2C12細胞移動速度百分比的分析方式……………………………19 　　伍、酵素免疫分析法(Enzyme-Linked Immunosorbent Assay, ELISA)……20 　　陸、統計分析………………………………………………………21 第四章　結果……………………………………………………………22 　第一節　小鼠C2C12肌原母細胞與肌小管細胞之型態………………22 　第二節　正常氧分壓下活化的吞噬細胞培養液 　　　　　對肌原母細胞移動速度的影響……………………………23 　第三節　低氧常壓環境下活化的吞噬細胞培養液 　　　　　對肌原母細胞移動速度的影響……………………………24 　第四節　正常氧分壓下活化的吞噬細胞培養液 　　　　　對肌小管細胞移動速度的影響……………………………25 　第五節　低氧常壓環境下活化的吞噬細胞培養液 　　　　　對肌小管細胞移動速度的影響……………………………26 　第六節　正常氧分壓下活化的吞噬細胞培養液 　　　　　中細胞激素的濃度…………………………………………27 　第七節　正常氧分壓下TNF-與IL-6對肌原母細胞 　　　　　之移動速度的影響…………………………………………28 　第八節　正常氧分壓下TNF-與IL-6對肌小管細胞 　　　　　之移動速度的影響…………………………………………29 第五章　討論……………………………………………………………30 參考文獻…………………………………………………………………49
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dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://ir.ntus.edu.tw/handle/987654321/71028
dc.languagezh-TW
dc.publisher競技運動學系碩士班
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dc.subject細胞激素;肌肉細胞;缺氧
dc.subjectcytokine;myogenic cell;hypoxia
dc.title探討活化的吞噬細胞對C2C12肌肉細胞移動速度的影響
dc.titleEFFECTS OF THE ACTIVATED MACROPHAGES ON THE MIGRATION OF C2C12 MYOGENIC CELLS
dc.typethesis
dspace.entity.typePublication

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學位論文(103學年度之前)