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建立篩選平台—影響肌原母細胞分化之化合物

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dc.contributor.advisor方世華
dc.contributor.advisorFang, Shih-Hua
dc.creator陳南君
dc.creatorChen, Nan-Chun
dc.date2010
dc.date.accessioned2017-02-27T06:29:09Z
dc.date.accessioned2025-07-30T15:30:01Z
dc.date.available2017-02-27T06:29:09Z
dc.date.issued2017-02-27T06:29:09Z
dc.description學位類別：碩士
dc.description校院名稱：國立台灣體育大學
dc.description系所名稱：運動健康科學系碩士班
dc.description學號：19706006
dc.description畢業學年度：98年
dc.description論文頁數：68頁
dc.description.abstract　　當運動員接觸到對肌肉有害的化合物，就會引發肌肉損傷的危機，影響其運動訓練與表現。我們希望利用一套嚴謹的偵測平台，提早評估可能影響肌肉發展及分化的成份。本研究論文以C2C12老鼠骨骼肌細胞作為實驗平台，將已知抑制肌細胞分化之氯化甲醇 (methoxychlor，MXC)當作為對照組，分析一系列“YMT”人工新合成的黃酮類化合物對肌細胞分化的影響，利用細胞型態、細胞存活率、肌肉特定蛋白Myogenin、肌酸激酶 (creatine kinase，CK)表現的變化作為評估的依據。實驗結果發現，不同的YMT新合成化合物對於肌細胞的影響也不相同，其中YMT10、12、14、16這4種藥物各別以0.5 nM、7.5 nM、1 nM、5 nM的濃度就可達到抑制50% 肌小管細胞存活率及細胞融合指數，遠比已知的MXC更具顯著的抑制效果。而且，藥物對分化後肌細胞內的肌酸激酶活性與Myogenin表現量的抑制作用也不相同。若在分化過程中加入YMT10、14此2種藥物後，發現分化後肌小管細胞的肌酸激酶活性、細胞融合指數及Myogenin蛋白質表現量皆會減少；然而在分化過程加入YMT12的肌小管細胞中發現，雖然細胞融合指數及肌酸激酶活性明顯減少，但Myogenin的蛋白質表現量並沒有明顯改變；另外，分化過程中加入YMT16藥物時，分化後的肌小管細胞之細胞融合指數及Myogenin 蛋白質表現量會減少，但肌酸激酶活性卻呈現增加趨勢。此外，在分化開始後的不同階段加入YMT10、12、14及16這4種藥物，發現藥物作用的時間即使未達3天，仍會對細胞分化產生抑制。由本實驗結果推測，YMT藥物的苯環結構所銜接的取代基可能造成對肌原母細胞及分化後的肌小管細胞作用濃度不同的原因之一。同時我們發現當肌原母細胞開始分化成肌小管細胞後，對藥物的敏感性也隨之改變。這4種不同藥物可能透過影響不同的細胞週期調控因子或訊號傳遞過程，進而改變了肌肉細胞分化後的各種特性。至於這些新合成化合物影響肌肉分化過程的分子機轉，未來需更進一步探討。目前本論文已建立一套篩選的技術平台，可以應用在篩選任何可能抑制或促進肌細胞分化的化合物。
dc.description.abstract　　If athletes contact harmful compounds, which may lead to muscle damage, and muscle injury will have impact on athletes training and performance. The aim of this study is to establish a rigorous screening platform for early detection of compounds that may exert inhibitory effects on muscle differentiation. We used C2C12 mouse myoblast cell line as an experimental model, and a synthetic known inhibitor of muscle cell differentiation-Methoxycholor (MXC) as positive control to monitor the accuracy of this screening platform. We investigated whether a series of newly synthesized flavonoids compounds, “YMT”, may affect the muscle cell differentiation. Meanwhile, we observed the changes of morphology, viability, muscle specific protein– Myogenin, and creatine kinase (CK) activity. The results showed that the effects a series of YMT compounds on C2C12 were different. When myoblasts were treated with 0.5 nM of YMT10, 7.5 nM of YMT12, 1.0 nM of YMT14, and 5.0 nM of YMT16, the cell viability and formation of myotube were decreased about 50% compared with the cell control. Interestingly, the effects of these YMT compounds on creatine kinase activity and Myogenin protein were different. YMT10, 12, 14 and16 were added to the myoblast cultures when shifted to the differentiation medium at the third day, YMT10 and 14 decreased the fusion index, creatine kinase activity and Myogenin；YMT12 decreased the fusion index and creatine kinase activity, but Myogenin did not change；YMT16 decreased the fusion index and Myogenin, but the creatine kinase activity was increased. In addition, YMT compounds effectively blocked the induction of fusion index when added at an early period of the differentiation of C2C12 cells. From our results, we can conclude that YMT analogues with different substituents might cause the different effects on myoblast or myotube. Meanwhile, we found the sensitivities of myoblast and myotube are different. YMT10, 12, 14 and16 may change the variety of characteristics through affecting the cell cycle regulator factors or signal transduction. However, further studies are needed to elucidate the detail mechanisms. In summary, we established a screening platform, which can be used to screen the effects of compounds on muscle cell differentiation.
dc.description.tableofcontents目　次 第壹章　引言……………………………………………………………1 　第一節　研究背景……………………………………………………1 　第二節　研究目的……………………………………………………3 　第三節　研究假設……………………………………………………4 　第四節　名詞解釋……………………………………………………5 第貳章　文獻探討………………………………………………………6 　第一節　肌肉細胞發育及分化起源…………………………………6 　第二節　肌肉損傷與免疫系統的關連………………………………10 　第三節　藥物對肌肉發育及分化影響………………………………17 　第四節　總結…………………………………………………………21 第參章　研究方法與步驟………………………………………………22 　第一節　實驗設計……………………………………………………22 　第二節　實驗材料與方法……………………………………………24 　　壹、細胞培養 (cell cu1ture)…………………………………………24 　　貳、氯化甲醇 (methoxychlor，MXC)及一系列YMT 　　　　新合成化合物對肌原母細胞的影響…………………………25 　　參、一系列YMT新合成化合物對肌小管細胞影響………………25 　　肆、結晶紫定量分析 (crystal violet test)25 　　伍、細胞型態的辨別—伊紅 (eosin-Y )染色法 　及細胞融合指數之計算……………………………………………26 　　陸、西方點墨法 (western blotting)偵測Myogenin 表現……………27 　　柒、肌酸激酶活性 (creatine kinase activity， 　　　　CK activity )之分析……………………………………………29 第肆章　結果……………………………………………………………30 　第一節　肌原母細胞與肌小管細胞之細胞型態……………………30 　第二節　不會直接造成肌原母細胞死亡的MXC及YMT 藥物濃度篩選………………………………………………………31 　第三節　YMT藥物造成分化後的肌小管細胞存活率 低於50%的濃度之測試……………………………………………32 　第四節　不同濃度的YMT10、YMT12、YMT14、 YMT16對肌小管細胞分化的影響…………………………………33 　第五節　肌原母細胞在不同濃度的YMT10、YMT12、 　　　　　YMT14、YMT16影響下分化成肌小管細胞 　　　　　中的肌酸激酶活性表現……………………………………34 　第六節　肌原母細胞在不同濃度的YMT10、YMT12、 YMT14、YMT16影響下分化成肌小管細胞中 的Myogenin蛋白質表現量………………………………………35 　第七節　不同分化階段加入YMT10、YMT12、YMT14、YMT16 　　　　　對肌小管細胞分化後的細胞融合指數之影響……………36 第伍章　討論……………………………………………………………37 參考文獻…………………………………………………………………51 　附錄……………………………………………………………………67
dc.format.extent1221962 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://ir.ntus.edu.tw/handle/987654321/70974
dc.languagezh-TW
dc.publisher運動健康科學系碩士班
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dc.subject細胞分化
dc.subjectcell differentiation
dc.title建立篩選平台—影響肌原母細胞分化之化合物
dc.titleTo Establish the Screen Platform —The Effects of Unknown Chemical Compounds On The Myoblasts Differentiation
dc.typethesis
dspace.entity.typePublication

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學位論文(103學年度之前)