Publication:

藉由茯苓多醣體在無熱壓力下脫水減重對大專男性角力運動員免疫調節的反應

本研究目的在探討大學角力運動員在無熱壓力下脫水減重對免疫系統的衝擊，招募25位男性大學角力運動員自願者經歷脫水計畫。研究方法：未避免影響培訓計畫，實驗時間選擇避開比賽期且沒有大強度的訓練、比賽或遊戲。實驗中訓練時間與強度為兩小時訓練中以25%（VO2peak）強度行走10分鐘並休息20分鐘，訓練期間隨時觀察體溫變化當體溫超過37.8度時即立刻停止訓練。在相同訓練環境下（溫度25.4 ± 3.3度,濕度89 ± 7 %，風速0.2 ± 0.1 m/s）依個別身體組成控制飲食能量需求以避免除了水分攝取限制之外因素影響實驗。13位控制組（DE）在84小時後尿液比重1.030，脫水減輕4.52％，其餘12位對照組（EU）無水分攝取限制。抽取血液分離周邊單核白血球（PBMNC）後以不同濃度茯苓多醣體共同培養（PCPS, 1-30 μg/l）24小時之後控制組（DE）對人類白血病細胞U937之成長抑制率，在濃度（PCPS, 25 μg/l）時達到最高峰。實驗結果：分析各組單核白血球（PBMNC）與茯苓多醣體（PCPS）共同培養（MNC-CM）後刺激人類白血病細胞U937，控制組脫水後的（PCPS-MNC-CM）的NBT還原試驗分析U937細胞被誘導分化成熟之程度，確實有較低的數值63.7±4.7%。並分析其脫水後的控制組的周邊單和白血球與茯苓多醣體的共同培養液POST-DE- PCPS-MNC-CM的細胞激素所分泌的INF-γ, IL-1β and TNF-α皆顯著低於對照組。結論：推論角力運動員在無熱壓力下脫水減重的免疫調節能力低於無脫水的對照組。

Background ：To evaluate the impact of hydration status under ambient environmental temperature on the immune system, 25 male collegiate wrestlers, were recruited to undergo dehydration program.Methods： To avoid the interference the training program and the performance, the experimental duration was selected to be not in season period , therefore, no subjects registered in any competitions, games or intensive training. The same mild exercise intensity （at about an individually estimated VO2peak of 25%）, as walking on treadmill, for 2 hour duration with 10 minutes rest with every 20 minutes intervals to measure tympanic body temperature to prevent from hyperthermia and the same water volume （1.5ml per Kcal） ingestion. When the Tympanic body temperature, measured at rest during exercise, was over 37.8 oC, the walking exercise on treadmill quits. 13 subjects, have controlled diets with individual energy requirements to prevent from body mass loss as well as with restricted water to cause 4.52% dehydration, as urinary specific gravity at about 1.030 in 84 hours, were dehydrated group （DE） and the others 12 subjects that of without water restriction to keep total body weight were euhydrated group （EU）. Peripheral blood monocytes （PBMNC） after dehydration was isolated to perform immunomodulatory response test by being incubated with polysaccharide fraction from fu-ling （Poria cocos）（PCPS, 1-30 μg/l）, which the conditioned medium are prepared to be termed as PCPS-MNC-CM. Greater PCPS （25 μg/l） was needed in DE group to prepare the PCPS-MNC-CM, which is reached up to plateau in growth inhibitory curve for Treated U973.Results： The Treated U937 cells, incubated together with POST-DE-PCPS-MNC-CM at post-dehydration, exhibited much lower NBT positive value as 63.7±4.7%. By analyzing the cytokines secretion in PCPS-MNC-CM from subjects after dehydration, amounts of INF-γ, IL-1β and TNF-α were much lower than that of euhydration.Conclusion： In conclusion, the immunomodulatory responsibilities by PCPS in dehydration were lower in male collegiate wrestlers.