This study was to investigate the effect of different stroke volume in badminton backcourt kill on physiology response, and further to realize different multi-balls training on energy utilization. Ten college badminton players (age 21.1±1.7 years, height 171.16±5.59 cm, and weight 65.19±7.86 kg) were recruited in this study. Average training year was 3.55±3.03 years. All Subjects were required to accomplished 10 balls a set (each set consisted of 10 balls, 4 set interval 30 s rest) and 5 balls a set (each set consisted of 5 balls, 8 set interval 30 s rest) training. Glucose and lactate level in baseline, during test, and end of test at 1, 2, 3, 7, 10, 15 min were determined. Heart rate was measured at baseline, whole test period; posttest immediately and 5 min post-test. The average of one set hitting time in ten-ball group was significantly more than five-ball group (24.2±2.23 v.s. 11.0±0.11 sec, p＜.05). Both two group exhibited higher heart rate in post-test immediately compared to the other tests, but it's not difference between 10 and 5 balls group. Both two group exhibited higher glucose level in per-test compared to the other tests, but it's not difference between 10 and 5 balls group. The level of lactate in ten-ball group was higher than in five-ball group at the beginning of test to post-test 7 min (2.95±0.2, 5.3±0.4, 6.29±0.48, 6.26±0.68, 5.92±0.65 v.s. 2.46±0.12, 3.11±0.25, 3.16±0.32, 2.9±0.34, 2.86±0.3mmol/l, p＜.05). The major finding in this study was that the multi-balls training of badminton backcourt kill in five-ball group was reasonable to reflect the energy utilization during competition.