Publication:

天然多酚對調節性細胞免疫反應之影響－-從系統生物學到分子免疫學

(Part I) 本研究針對跆拳道選手在高強度的訓練後攝取綠茶對於黏膜免疫、唾液中抗菌蛋白表現及抗氧化能力之影響。22位跆拳道選手在進行2小時的高強度訓練後，攝取綠茶或是水當作對照組。分別在訓練前、訓練後及喝完綠茶後30分鐘收集唾液，進行分析。結果發現澱粉酶活性，乳鐵蛋白及免疫球蛋白A 的濃度在高強度訓練後立即顯著增加，但是抗菌能力沒有明顯的改變。如果攝取綠茶者其抗菌能力及澱粉酶活性顯著增加，澱粉酶活性及免疫球蛋白A在休息30分鐘後都回到與訓練前差不多。另外我們發現唾液中抗氧化能力在訓練後立即下降但休息後又回到與訓練前的水準。我們發現攝取綠茶對抗菌能力有顯著的增強。 (Part II) 本研究針對舉重選手在連續高強度的訓練後對黏膜免疫系統功能之影響。11位優秀的選手分別在比賽前三個不同的階段與比賽後休息的階段分別收集唾液，進行分析。結果發現唾液中總蛋白質的量不受影響；訓練期的免疫球蛋白A、免疫球蛋白A對總蛋白質的比值、可體松及可體松對總蛋白質的比值顯著高過休息的階段；在免疫球蛋白A對總蛋白質的比值與可體松對總蛋白質的比值有顯著正相關的關係。這些發現顯示長時間、高強度的阻力訓練將影響舉重選手的免疫球蛋白A與可體松的反應。

(Part I) This study was aimed to investigate the acute effects of green tea consumption on selected salivary defense proteins, antibacterial capacity and anti-oxidation activity in taekwondo (TKD) athletes following intensive training. Twenty-two TKD athletes performed a 2-hr TKD training. After exercise, participants ingested green tea or equal volume of water. Saliva samples were collected before training, immediately after training but before drinking, and 30 min after drinking green tea or water. Results show that α-amylase activity and lactoferrin, sIgA concentrations were significantly increased immediately after intensive TKD training, however, salivary antibacterial capacity was not affected by intense training. Green tea consumption after training enhanced salivary antibacterial capacity and further stimulated α-amylase activity. Lactoferrin and sIgA concentrations returned to pre-exercise values after 30 min of rest. Additionally, we observed that salivary FRSA was markedly suppressed immediately after training and quickly returned to pre-exercise values regardless of which fluid was consumed. Our results demonstrated that green tea consumption exerts acute effect on the concentrations of salivary oral defense-related proteins and significantly enhances salivary antibacterial capacity. (Part II) The aim of this study was to investigate the cumulative effects of intensive resistance training on salivary immunoglobulin A (SIgA) and cortisol responses in elite male weightlifters. Eleven elite male Taiwanese weightlifters trained through three training stages before a national weightlifting competition and followed by a two-week recovery stage. Results showed (a) salivary TP concentrations were not significantly affected; (b) resting levels of SIgA, SIgA/TP, cortisol, and cortisol/TP were significantly higher in training stages than in recovery stage; (c) a positive correlation was revealed between the ratios of SIgA/TP and cortisol/TP; and (d) the resting salivary lactoferrin concentrations and the ratio of lactoferrin/TP were significantly lower in stage I than in recovery stage. The findings in this study suggest that prolonged, intensive resistance training exerts cumulative effects on SIgA and cortisol responses in elite weightlifters.