Publication: Bitter melon seed oil increases mitochondrial content in gastrocnemius muscle and improves running endurance in sedentary C57BL/6J mice
| dc.creator | Chan, Fei Koon;Hsu, Chin;Li, Tsai-Chung;Chen, Wen-Hung;Tseng, Kuo-Tang;Chao, Pei-Min | |
| dc.date | 2018-08 | |
| dc.date.accessioned | 2020-01-03T08:58:26Z | |
| dc.date.accessioned | 2025-07-28T15:04:17Z | |
| dc.date.available | 2020-01-03T08:58:26Z | |
| dc.date.issued | 2020-01-03T08:58:26Z | |
| dc.description.abstract | The α-eleostearic acid (α-ESA) in bitter melon seed oil (BMSO) is efficiently converted by the body into rumenic acid. The objective of this study was to investigate effects of BMSO on skeletal muscle fiber-type switch and endurance capacity in mice, with or without exercise training. In a 3×2 factorial design, C57BL/6J mice were fed a 30% high-fat diet composed of soybean oil, butter or a 1:1 mixture of BMSO and soybean oil, i.e., SB, BT and BM diets, respectively, and were allocated to be sedentary or undergo exercise (Ex). The Ex groups received a 15-min training regimen on a motorized treadmill 5 times a week. After 3-week intervention, endurance capacity was evaluated (total running time and distance until exhaustion). Mice fed a BM diet had significantly less body fat, with increased muscle percentage and improved endurance capacity. Combining sedentary and Ex groups, mice fed a BM diet ran 33% longer and 50% further than those fed SB, or 25% longer and 36% further than those fed BT (P<.01). The BM-diet-increased gastrocnemius cytochrome c protein and mitochondrial DNA content was more prominent in sedentary than in trained mice. Histochemical staining shows sedentary BM-fed mice had a higher succinate dehydrogenase activity among groups. Based on a reporter assay, rumenic acid, rather than α-ESA itself, activated PPARδ ligand binding domain. We concluded that BMSO improved endurance capacity via stimulation of mitochondrial biogenesis and function, potentially influencing muscle metabolism and fiber-type composition in sedentary mice. | |
| dc.format.extent | 137 bytes | |
| dc.format.mimetype | text/html | |
| dc.identifier.uri | https://ir.ntus.edu.tw/handle/987654321/65566 | |
| dc.language | zh_TW | |
| dc.publisher | Elsevier B.V. | |
| dc.relation | The Journal of Nutritional Biochemistry, Vol.58, pp.150-157 | |
| dc.subject | Bitter melon seed oil; α-ESA; Rumenic Acid; Exercise Endurance; Mitochondri | |
| dc.title | Bitter melon seed oil increases mitochondrial content in gastrocnemius muscle and improves running endurance in sedentary C57BL/6J mice | |
| dc.type | article | |
| dspace.entity.type | Publication |