近年來長時間的耐力型運動吸引越來越多人的參與，例如多天期的自行車活動， 且參予者包含男女性以及廣泛的年齡層，這些未受訓練者對此種運動型態可能具 有不同的生理反應，但目前仍缺乏探討不同性別與年齡的未受訓練者，於此類運 動的生理與生物力學反應之相關研究。同時，大多數研究僅探討運動後短時間內 的生理變化，因此，本二年期的整合型研究計畫將探討未受訓練者於15 天單車 環島期間與結束後14 天的生理與生物力學變化，並探討這些變化的性別與年齡 差異，第一年將著重於性別差異，第二年則探討年齡差異。本整合型計畫將包含 以下子計畫: (1)凝血與纖維蛋白分解指標; (2)心肺及肌肉生理; (3)免疫系統; (4) 同化性與異化性荷爾蒙; (5)骨骼代謝指標; (6)神經肌肉疲勞機轉。將於環島期間 第1, 3, 6, 9, 12, 15 日，及環島結束後第3, 7, 14 日採集早晨空腹血液、尿液與唾 液樣本，並於環島前3-5 日，環島後3-5 日，及環島後14-16 日進行心肺與神經 肌肉測試。本整合型研究計畫將可瞭解多種生理系統於多天期耐力型運動期間與 之後的變化情形，並可暸解各系統變化之間的相互關係，本研究結果可全面性的 提升參與者於運動期間及結束後的健康，使他們更可享受這種運動。
Purpose: Intensive exercise may increase free radical production. Multi-day cycling tours have been gaining significant popularity in recent years. The aims of the present study were to investigate the changes in oxidative stress markers, plasma malondialdehyde (MDA) and oxidized LDL (oxLDL) levels, and plasma lipid profile, including total cholesterol (TC), LDL-cholesterol, HDL-cholesterol, and triglyceride, during a 15-day 1100-km cycling tour in untrained healthy subjects. Methods: Eighteen healthy subjects (9 males and 9 females) who participated in Cycling Taiwan 1000 km were recruited. The tour covered approximately 1100 km in 15 days. Fasting blood samples were collected in the early morning on day 1, 4, 8, 12, and 15 during the tour. Results: Plasma MDA concentrations were significantly lower in day 8 (0.47±0.20 M) and 12 (0.54±0.16 M) compared to day 1 (0.93±0.39 M), then returned to the baseline level at the end of the tour. Plasma oxLDL concentrations were increased slightly until day 12, then dropped to the level significantly lower than the baseline (53.69±17.45 U/L) on day 15 (43.35±12.61 U/L). TC was significantly decreased on day 4 and 8 (4.16±0.68 and 3.83±0.58 mM, respectively), then returned to the baseline level on day 12 and 15 (4.43±0.59 and 4.19±0.51 mM, respectively). The changes in TC concentrations resulted mostly from the changes in LDL-Cholesterol. Conclusions: The current study suggested that 15 days of endurance exercise did not result in higher oxidative stress at the end of the tour in untrained male and female subjects. In addition, the tour only had minor effect on plasma lipid profile in these subjects.