Publication:

藉由單核球細胞免疫反應抑制人類B型肝炎病毒表面抗原作用探討老年人之自行車運動對免疫力提升效果之研究

研究背景在研究具習慣性自行車運動的老年人(HCE，Habitual cycling exercise)藉由每日清晨低強度的運動，經實驗控制，與對照組(SC，Sedentary control)相比之下，而表現出較高抗B型肝炎病毒的免疫活性。其方法利用植物血凝集素(PHA，Phytohemagglutin)刺激後表現之人類週邊血單核球細胞加入可表現B型肝炎病毒的Hep-3B細胞培養基下，觀察免疫抑制情形。

結果發現為經由實驗設計與控制後的受試者表現情形，在自行車運動計劃過程中觀察到VO2 max最高峰可達到43.54%，HR max可達到60.12%。因此認為HCE對於老年人是屬於一種有氧之中低強度運動。在此過程中所表現出之細胞激素(Cytokine)像是IFN-γ，TNF-α和IFN-α，在培養基的表現情形，具有運動習慣之HCE組較優於SC組。而在相同濃度的PHA刺激之下，觀察兩者對於抑制B型肝炎病毒的表現，其中HCE為64.7%，而SC為81.5%。然而下降的抑制率在細胞激素中和試驗下，可以推測其關鍵的證據是由於細胞激素的作用而造成HCE-MNC-CM對於HBS Ag表現抑制情形。

結論為透過數據顯示出具自行車運動習慣的老年人與正常無運動習慣的老年人相比下，觀察經由PHA刺激B型肝炎病毒表面抗原表現過程中之單核球細胞免疫反應情形，HCE組較優於SC組。

Background:Whether elderly with habitual cycling exercise (HCE), at lower intensity in the morning, have higher immunity against hepatitis B virus than sedentary controls (SC) was a health care issue for elderly.Methods:The conditioned media were prepared by stimulating the isolated human peripheral blood mononuclear cells with phytohemagglutinin (PHA) for assessment of the inhibitory effects on hepatitis B surface antigen (HBsAg) expression in human hepatoma Hep3B cells.

Results:By the detected % VO2peak at about 43.54 % and % HRmax at about 60.12% during cycling exercise program in present study, we can considered HCE as an aerobic and low to moderate exercise for elderly. The concentrations of secreted cytokines such as IFN-γ, TNF-α and IFN-α were greater in the MNC-CM from the HCE group than those from the SC group. The inhibitory rates of HCE group’MNC-CM against the HBsAg expression were greater than that of SC group. In the same stimulating concentration of PHA (at 10 μg/ml), the relative HBsAg expression in HCE group’ MNC-CM was 64.7 % versus 81.5 % of SC group. The decline of inhibitory rates in cytokine neutralization experiments suggest that the crucial roles played by these cytokines for inhibitory effect of HCE-MNC-CM against HbsAg expression.

Conclusion:The results reveal that the immune response of MNC, which are stimulated with PHA to suppress HBsAg expression in elderly with habitual cycling exercise at lower speed, is greater than that in sedentary controls.